Upgrading protein synthesis for synthetic biology

Nat Chem Biol. 2013 Oct;9(10):594-8. doi: 10.1038/nchembio.1339.

Abstract

Genetic code expansion for synthesis of proteins containing noncanonical amino acids is a rapidly growing field in synthetic biology. Creating optimal orthogonal translation systems will require re-engineering central components of the protein synthesis machinery on the basis of a solid mechanistic biochemical understanding of the synthetic process.

MeSH terms

  • Amino Acids / chemistry
  • Amino Acids / genetics
  • Amino Acids / metabolism
  • Genetic Code / genetics
  • Molecular Conformation
  • Protein Biosynthesis / genetics*
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / metabolism*
  • RNA, Transfer / chemistry
  • RNA, Transfer / metabolism
  • Synthetic Biology*

Substances

  • Amino Acids
  • Proteins
  • RNA, Transfer