Analysis of Y chromosome haplogroups in Japanese population using short amplicons and its application in forensic analysis

Yuta Harayama; Sayako Kamei; Noriko Sato; Tokutaro Hayashi; Tetsuya Shiozaki; Masao Ota; Hideki Asamura

doi:10.1016/j.legalmed.2013.10.005

Analysis of Y chromosome haplogroups in Japanese population using short amplicons and its application in forensic analysis

Leg Med (Tokyo). 2014 Jan;16(1):20-5. doi: 10.1016/j.legalmed.2013.10.005. Epub 2013 Nov 1.

Authors

Yuta Harayama¹, Sayako Kamei², Noriko Sato², Tokutaro Hayashi², Tetsuya Shiozaki², Masao Ota², Hideki Asamura²

Affiliations

¹ Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan. Electronic address: 10mh027c@shinshu-u.ac.jp.
² Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, Matsumoto, Nagano 390-8621, Japan.

PMID: 24262653
DOI: 10.1016/j.legalmed.2013.10.005

Abstract

We designed three mini multiplex PCR systems using single-base extension reactions to identify Japanese Y chromosome haplogroups. We selected a group of 22 Y chromosome single nucleotide polymorphisms (SNPs) from the haplogroups most commonly reported in East Asia. To make the systems more useful in analyzing degraded DNA samples, we designed primers to render amplicons of ≤ 150 bp. Applying these systems, we classified the Japanese population into major haplogroups and confirmed the applicability of these systems in forensic DNA analysis.

Keywords: Degraded DNA; Multiplex single base extension reactions; Short PCR products; Y chromosome SNPs.

MeSH terms

Asian People / genetics*
Chromosomes, Human, Y*
Forensic Genetics / methods*
Genetics, Population
Haplotypes
Humans
Male
Nucleic Acid Amplification Techniques
Polymerase Chain Reaction
Polymorphism, Single Nucleotide