Near-infrared-fluorescence imaging of lymph nodes by using liposomally formulated indocyanine green derivatives

Taro Toyota; Hiromichi Fujito; Akiko Suganami; Tomoki Ouchi; Aki Ooishi; Akira Aoki; Kazutaka Onoue; Yutaka Muraki; Tomoyuki Madono; Masanori Fujinami; Yutaka Tamura; Hideki Hayashi

doi:10.1016/j.bmc.2013.12.026

Near-infrared-fluorescence imaging of lymph nodes by using liposomally formulated indocyanine green derivatives

Bioorg Med Chem. 2014 Jan 15;22(2):721-7. doi: 10.1016/j.bmc.2013.12.026. Epub 2013 Dec 20.

Authors

Affiliations

¹ Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan; Department of Bioinformatics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan; Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan.
² Department of Medical System Engineering, Faculty of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
³ Department of Bioinformatics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
⁴ Division of Nanoscience, Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
⁵ Yamada Chemical Co. Ltd, 1-1 Kamichoshi-cho, Kamitoba, Minami-ku, Kyoto 601-8105, Japan.
⁶ Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
⁷ Center for Frontier Medical Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan; Department of Frontier Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. Electronic address: hhayashi@faculty.chiba-u.jp.

PMID: 24393719
DOI: 10.1016/j.bmc.2013.12.026

Abstract

Liposomally formulated indocyanine green (LP-ICG) has drawn much attention as a highly sensitive near-infrared (NIR)-fluorescence probe for tumors or lymph nodes in vivo. We synthesized ICG derivatives tagged with alkyl chains (ICG-Cn), and we examined NIR-fluorescence imaging for lymph nodes in the lower extremities of mice by using liposomally formulated ICG-Cn (LP-ICG-Cn) as well as conventional liposomally formulated ICG (LP-ICG) and ICG. Analysis with a noninvasive preclinical NIR-fluorescence imaging system revealed that LP-ICG-Cn accumulates in only the popliteal lymph node 1h after injection into the footpad, whereas LP-ICG and ICG accumulate in the popliteal lymph node and other organs like the liver. This result indicates that LP-ICG-Cn is a useful NIR-fluorescence probe for noninvasive in vivo bioimaging, especially for the sentinel lymph node.

Keywords: Indocyanine green (ICG); Liposome; Lymph node; Near-infrared (NIR)-fluorescence probe; Phospholipid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diagnostic Imaging*
Fluorescence*
Fluorescent Dyes* / chemistry
Indocyanine Green* / chemistry
Liposomes / chemistry*
Lymph Nodes / pathology*
Male
Mice
Mice, Inbred Strains
Molecular Structure
Rats
Rats, Sprague-Dawley
Spectrometry, Fluorescence
Spectroscopy, Near-Infrared

Substances

Fluorescent Dyes
Liposomes
Indocyanine Green