Purification and identification of a cytokinin from moss callus cells

P Beutelmann; L Bauer

doi:10.1007/BF00380679

Purification and identification of a cytokinin from moss callus cells

Planta. 1977 Jan;133(3):215-7. doi: 10.1007/BF00380679.

Authors

P Beutelmann¹, L Bauer

Affiliation

¹ MSU/ERDA Plant Research Laboratory, 48824, East Lansing, MI, USA.

PMID: 24425252
DOI: 10.1007/BF00380679

Abstract

A cytokinin was isolated from the culture medium of callus cells of the moss hybridFunaria hygrometrica (L.) Sibth xPhyscomitrium piriforme Brid. The purification procedure included ethyl-acetate extraction, silver-salt precipitation, crystallization as picrate, and ion exchange chromatography. The structure of the cytokinin was confirmed as N(6)-(Δ(2)-isopentenyl)adenine by means of gas chromatography and mass spectrometry. The concentration of the compound in the culture medium was determined at ca. 10(-6) M.