NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain

Bytul Rahman; Shin Kawano; Kaori Yunoki-Esaki; Takahiro Anzai; Toshiya Endo

doi:10.1016/j.febslet.2013.12.037

NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain

FEBS Lett. 2014 Mar 3;588(5):678-84. doi: 10.1016/j.febslet.2013.12.037. Epub 2014 Jan 23.

Authors

Bytul Rahman¹, Shin Kawano¹, Kaori Yunoki-Esaki², Takahiro Anzai¹, Toshiya Endo³

Affiliations

¹ Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
² Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan; JST, CREST, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
³ Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan; JST, CREST, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan; Structural Biology Research Center, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan. Electronic address: endo@chem.nagoya-u.ac.jp.

PMID: 24462684
DOI: 10.1016/j.febslet.2013.12.037

Abstract

The mitochondrial targeting signal in the presequence of mitochondrial precursor proteins is recognized by Tom20 and subsequently by Tim50 in mitochondria. Yeast Tim50 contains two presequence binding sites in the conserved core domain and in the fungi-specific C-terminal presequence binding domain (PBD). We report the NMR analyses on interactions of a shorter variant of PBD (sPBD), a shorter variant of PBD, with presequences. The presequence is recognized by sPBD in a similar manner to Tom20. sPBD can also bind to the core domain of Tim50 through the presequence binding region, which could promote transfer of the presequence from sPBD to the core domain in Tim50.

Keywords: NMR; Presequence recognition; Tim50; Tom20; Yeast.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amino Acid Substitution
Binding Sites
Hydrophobic and Hydrophilic Interactions
Magnetic Resonance Spectroscopy
Mitochondrial Membrane Transport Proteins / chemistry*
Mitochondrial Membrane Transport Proteins / genetics
Mitochondrial Precursor Protein Import Complex Proteins
Molecular Sequence Data
Protein Binding
Protein Interaction Domains and Motifs
Protein Interaction Mapping
Protein Sorting Signals
Saccharomyces cerevisiae Proteins / chemistry*
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae*

Substances

Mitochondrial Membrane Transport Proteins
Mitochondrial Precursor Protein Import Complex Proteins
Protein Sorting Signals
Saccharomyces cerevisiae Proteins
TIM50 protein, S cerevisiae