P300 binds to and acetylates MTA2 to promote colorectal cancer cells growth

Biochem Biophys Res Commun. 2014 Feb 14;444(3):387-90. doi: 10.1016/j.bbrc.2014.01.062. Epub 2014 Jan 24.

Abstract

MTA2 is a member of metastasis associated family, which is highly expressed in several solid tumors and associated with tumor cells migration and invasion. Here, we report that MTA2 is acetylated at K152 and histone acetyltransferase p300 binds to and acetylates MTA2. Furthermore, mutation of the MTA2 acetylation site inhibits the growth of colorectal cancer cells and migration and invasion of Rat1 fibroblasts. These results reveal a novel post-translational regulation of MTA2 by the way of p300-dependent acetylation, which is important for tumor cells growth and migration and provides a potential target for clinical cancer research.

Keywords: Acetylation; Cell growth; MTA2; Migration; P300.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Amino Acid Sequence
  • Animals
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / pathology*
  • E1A-Associated p300 Protein / metabolism*
  • Histone Deacetylases / chemistry
  • Histone Deacetylases / metabolism*
  • Humans
  • Molecular Sequence Data
  • Protein Binding
  • Repressor Proteins / chemistry
  • Repressor Proteins / metabolism*
  • Sequence Homology, Amino Acid

Substances

  • Repressor Proteins
  • E1A-Associated p300 Protein
  • EP300 protein, human
  • MTA2 protein, human
  • Histone Deacetylases