Draft genome sequence of Xanthomonas axonopodis pv. glycines 8ra possessing transcription activator-like effectors used for genetic engineering

Ju-Hoon Lee; Hakdong Shin; Hye-Jee Park; Sangryeol Ryu; Sang-Wook Han

doi:10.1016/j.jbiotec.2014.03.009

Draft genome sequence of Xanthomonas axonopodis pv. glycines 8ra possessing transcription activator-like effectors used for genetic engineering

J Biotechnol. 2014 Jun 10:179:15-6. doi: 10.1016/j.jbiotec.2014.03.009. Epub 2014 Mar 20.

Authors

Ju-Hoon Lee¹, Hakdong Shin², Hye-Jee Park³, Sangryeol Ryu², Sang-Wook Han⁴

Affiliations

¹ Department of Food Science and Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea.
² Department of Food and Animal Biotechnology, and Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
³ Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea.
⁴ Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Republic of Korea. Electronic address: swhan@cau.ac.kr.

PMID: 24657734
DOI: 10.1016/j.jbiotec.2014.03.009

Abstract

Xanthomonas axonopodis pv. glycines 8ra is a causal agent of bacterial pustule disease in soybean. This bacterium possesses transcription activator-like (TAL) effectors which are useful for genetic/protein engineering applications in higher organisms including plants and humans. Here, we report that the draft genome sequence consists of 5,337,885-bp double-stranded DNA encoding 4674 open reading frames (ORFs) in 13 different contigs. This genome sequence would be useful in applications of TAL effectors in genetic engineering and in elucidating virulence factors against plants.

Keywords: Genome; Pathogen; Transcription activator-like (TAL) effectors; Xanthomonas axonopodis pv. glycines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Genetic Engineering
Genome, Bacterial*
Glycine max / microbiology
Molecular Sequence Data
Open Reading Frames
Sequence Analysis, DNA
Virulence Factors / genetics
Xanthomonas axonopodis / classification
Xanthomonas axonopodis / genetics*
Xanthomonas axonopodis / metabolism

Substances

Bacterial Proteins
Virulence Factors

Associated data

GENBANK/JDSU00000000