Clear cell papillary renal cell carcinoma: micro-RNA expression profiling and comparison with clear cell renal cell carcinoma and papillary renal cell carcinoma

Enrico Munari; Luigi Marchionni; Apurva Chitre; Masamichi Hayashi; Guido Martignoni; Matteo Brunelli; Stefano Gobbo; Pedram Argani; Mohamad Allaf; Mohammad O Hoque; George J Netto

doi:10.1016/j.humpath.2014.01.013

Clear cell papillary renal cell carcinoma: micro-RNA expression profiling and comparison with clear cell renal cell carcinoma and papillary renal cell carcinoma

Hum Pathol. 2014 Jun;45(6):1130-8. doi: 10.1016/j.humpath.2014.01.013. Epub 2014 Jan 31.

Authors

Affiliations

¹ Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.
² Department of Oncology, Johns Hopkins University, Baltimore, MD 21287, USA.
³ Department of Otolaryngology, Johns Hopkins University, Baltimore, MD 21287, USA.
⁴ Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy; Anatomic Pathology, Pederzoli Hospital, Peschiera d/G, Verona 37019, Italy.
⁵ Department of Pathology and Diagnostics, University of Verona, Verona 37134, Italy.
⁶ Department of Urology, Johns Hopkins University, Baltimore, MD 21287, USA.
⁷ Department of Otolaryngology, Johns Hopkins University, Baltimore, MD 21287, USA. Electronic address: mhoque1@jhmi.edu.
⁸ Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA; Department of Oncology, Johns Hopkins University, Baltimore, MD 21287, USA; Department of Urology, Johns Hopkins University, Baltimore, MD 21287, USA. Electronic address: gnetto1@jhmi.edu.

Abstract

Clear cell papillary renal cell carcinoma (CCPRCC) is a low-grade renal neoplasm with morphological characteristics mimicking both clear cell renal cell carcinoma (CCRCC) and papillary renal cell carcinoma (PRCC). However, despite some overlapping features, their morphological, immunohistochemical, and molecular profiles are distinct. Micro-RNAs (miRNAs) are small noncoding RNAs that play a crucial role in regulating gene expression and are involved in various biological processes, including cancer development. To better understand the biology of this tumor, we aimed to analyze the miRNA expression profile of a set of CCPRCC using microarray and quantitative reverse transcription-polymerase chain reaction. A total of 15 cases diagnosed as CCPRCC were used in this study. Among the most differentially expressed miRNA in CCPRCC, we found miR-210, miR-122, miR-34a, miR-21, miR-34b*, and miR-489 to be up-regulated, whereas miR-4284, miR-1202, miR-135a, miR-1973, and miR-204 were down-regulated compared with normal renal parenchyma. To identify consensus of differentially regulated miRNA between CCPRCC, CCRCC, and PRCC, we additionally determined differential miRNA expression using 2 publically available microarray data sets from the NCBI Gene Expression Omnibus database (GSE41282 and GSE3798). This comparison revealed that the miRNA expression profile of CCPRCC shows some overlapping characteristics between CCRCC and PRCC. Moreover, CCPRCC lacks dysregulation of important miRNAs typically associated with aggressive behavior. In summary, we describe the miRNA expression profile of a relatively infrequent type of renal cancer. Our results may help in understanding the molecular underpinning of this newly recognized entity.

Keywords: Clear cell papillary renal cell carcinoma; Micro-RNA; Profiling; Renal cancer.

Publication types

Comparative Study

MeSH terms

Adult
Aged
Carcinoma, Papillary / genetics*
Carcinoma, Papillary / pathology
Carcinoma, Renal Cell / genetics*
Carcinoma, Renal Cell / pathology
Female
Gene Expression Profiling
Humans
Kidney Neoplasms / genetics*
Kidney Neoplasms / pathology
Male
MicroRNAs / genetics*
Middle Aged
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Transcriptome*

Substances

MicroRNAs

Grants and funding

P30 CA006973/CA/NCI NIH HHS/United States