Banded structures in collagen vitrigels for corneal injury repair

Zhiyong Xia; Xiomara Calderón-Colón; Russell McCally; Jeffrey Maranchi; Lixia Rong; Benjamin Hsiao; Jennifer Elisseeff; Morgana Trexler

doi:10.1016/j.actbio.2014.05.010

Banded structures in collagen vitrigels for corneal injury repair

Acta Biomater. 2014 Aug;10(8):3615-9. doi: 10.1016/j.actbio.2014.05.010. Epub 2014 May 22.

Authors

Zhiyong Xia¹, Xiomara Calderón-Colón², Russell McCally³, Jeffrey Maranchi², Lixia Rong⁴, Benjamin Hsiao⁵, Jennifer Elisseeff⁶, Morgana Trexler⁷

Affiliations

¹ The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA. Electronic address: zhiyong.xia@jhuapl.edu.
² The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA.
³ The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA; The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
⁴ State University of New York at Stony Brook, Stony Brook, NY 11794-3400, USA.
⁵ The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
⁶ The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA; The Johns Hopkins University, Department of Biomedical Engineering, Baltimore, MD 21231, USA.
⁷ The Johns Hopkins University, Applied Physics Laboratory, Laurel, MD 20723, USA. Electronic address: morgana.trexler@jhuapl.edu.

PMID: 24859294
DOI: 10.1016/j.actbio.2014.05.010

Abstract

There is a growing interest in using collagen vitrigels for corneal injury repair. We recently reported the synthesis and thermal denaturation behavior of these gels. In this paper, the banded structure in these vitrified gels is studied by small-angle X-ray scattering (SAXS) one-dimensional (1-D) correlation function analysis and transmission electron microscopy (TEM). Results demonstrate that the collagen vitrigel possess banded structures similar to those of the starting type I collagen, with an average D-spacing of 64nm (by SAXS) or 57nm (by TEM). A combination of SAXS 1-D correlation function analyses and TEM show that overlap and gap distances ranged from 30 to 33nm and from 23 to 25nm, respectively. Changing the vitrification condition does not impact on the banded structure significantly.

Keywords: Banded structure; Corneal injury repair; D-Spacing; Small-angle X-ray scattering; collagen vitrigel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomimetic Materials / chemistry*
Cattle
Collagen / chemistry*
Corneal Injuries / therapy*
Humans
Hydrogels / chemistry*
Materials Testing
Microscopy
Microscopy, Electron, Transmission
Molecular Conformation
Scattering, Small Angle
Tissue Scaffolds*
Vitrification
X-Ray Diffraction

Substances

Hydrogels
Collagen