Abstract
The pancreatic stone protein isolated from human calculi (PSP) derives from the immunoreactive protein forms detected in human pancreatic juice (PSP S2-5) through the tryptic cleavage of the Arg-11-Ile-12 bond. Among the eleven amino acids of the PSP S2-5 N-terminal extension Z-E-A-Q-T-E-L-P-Q-A-R, the first residue is an oxoproline and the fifth, a threonine, bears the single carbohydrate chain of the protein molecules. Variations in the glycan chain composition account for the differences in the Mr of PSP S2-5. The PSP S2-5 forms are very soluble in aqueous solutions between the pH values 5.0-9.0, whereas the proteolysated form is scarcely soluble.
MeSH terms
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Acetylgalactosamine / analysis
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Acetylglucosamine / analysis
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Amino Acid Sequence
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Amino Acids / analysis
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Calcium-Binding Proteins* / analysis
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Calcium-Binding Proteins* / metabolism
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Carbohydrates / analysis
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Glycosylation
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Humans
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Hydrogen-Ion Concentration
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Lithostathine
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Molecular Sequence Data
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Nerve Tissue Proteins*
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Pancreatic Juice / analysis
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Peptide Fragments / metabolism
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Phosphoproteins
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Pyrrolidinones*
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Pyrrolidonecarboxylic Acid*
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Solubility
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Threonine / metabolism*
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Trypsin / metabolism
Substances
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Amino Acids
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Calcium-Binding Proteins
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Carbohydrates
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Lithostathine
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Nerve Tissue Proteins
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Peptide Fragments
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Phosphoproteins
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Pyrrolidinones
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REG1A protein, human
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Threonine
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Trypsin
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Acetylgalactosamine
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Pyrrolidonecarboxylic Acid
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Acetylglucosamine