Label-free, in situ SERS monitoring of individual DNA hybridization in microfluidics

Nanoscale. 2014 Aug 7;6(15):8521-6. doi: 10.1039/c4nr01951b.

Abstract

We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Breast Neoplasms / diagnosis
  • Breast Neoplasms / metabolism
  • Carbocyanines / chemistry
  • DNA / chemistry*
  • DNA, Single-Stranded / chemistry
  • Diffusion
  • Female
  • Gold / chemistry*
  • Humans
  • Light
  • Metal Nanoparticles / chemistry
  • Microfluidic Analytical Techniques*
  • Microfluidics
  • Nanostructures / chemistry
  • Nanotechnology
  • Nucleic Acid Hybridization*
  • Porosity
  • Receptor, ErbB-2 / chemistry
  • Spectrum Analysis, Raman*
  • Surface Plasmon Resonance

Substances

  • Carbocyanines
  • DNA, Single-Stranded
  • cyanine dye 3
  • Gold
  • DNA
  • ERBB2 protein, human
  • Receptor, ErbB-2