Abstract
We present label-free, in situ monitoring of individual DNA hybridization in microfluidics. By immobilizing molecular sentinel probes on nanoporous gold disks, we demonstrate sensitivity approaching the single-molecule limit via surface-enhanced Raman scattering which provides robust signals without photobleaching for more than an hour. We further demonstrate that a target concentration as low as 20 pM can be detected within 10 min under diffusion-limited transport.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Breast Neoplasms / diagnosis
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Breast Neoplasms / metabolism
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Carbocyanines / chemistry
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DNA / chemistry*
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DNA, Single-Stranded / chemistry
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Diffusion
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Female
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Gold / chemistry*
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Humans
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Light
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Metal Nanoparticles / chemistry
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Microfluidic Analytical Techniques*
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Microfluidics
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Nanostructures / chemistry
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Nanotechnology
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Nucleic Acid Hybridization*
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Porosity
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Receptor, ErbB-2 / chemistry
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Spectrum Analysis, Raman*
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Surface Plasmon Resonance
Substances
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Carbocyanines
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DNA, Single-Stranded
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cyanine dye 3
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Gold
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DNA
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ERBB2 protein, human
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Receptor, ErbB-2