Improved performance of the PacBio SMRT technology for 16S rDNA sequencing

J Microbiol Methods. 2014 Sep:104:59-60. doi: 10.1016/j.mimet.2014.06.012. Epub 2014 Jun 27.

Abstract

Improved sequencing accuracy was obtained with 16S amplicons from environmental samples and a known pure culture when upgraded Pacific Biosciences (PacBio) hardware and enzymes were used for the single molecule, real-time (SMRT) sequencing platform. The new PacBio RS II system with P4/C2 chemistry, when used with previously constructed libraries (Mosher et al., 2013) surpassed the accuracy of Roche/454 pyrosequencing platform. With accurate read lengths of >1400 base pairs, the PacBio system opens up the possibility of identifying microorganisms to the species level in environmental samples.

Keywords: 16S; PacBio; SMRT sequencing.

Publication types

  • Evaluation Study

MeSH terms

  • DNA, Bacterial / genetics*
  • DNA, Ribosomal / genetics*
  • High-Throughput Nucleotide Sequencing / instrumentation
  • High-Throughput Nucleotide Sequencing / methods*
  • RNA, Ribosomal, 16S / genetics*
  • Shewanella / genetics*
  • Shewanella / isolation & purification

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S