The glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) is regulated by myeloid zinc finger 1 (MZF-1) and is induced by calcitriol

Biochem Biophys Res Commun. 2014 Aug 15;451(1):137-41. doi: 10.1016/j.bbrc.2014.07.082. Epub 2014 Jul 24.

Abstract

Recently, new tissue-specific functions for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) have been discovered, aside from its archetypal function in glycolysis. This casts doubt on the legitimacy of using GAPDH as a normalization control for gene expression analysis. We report the binding of the myeloid zinc finger-1 (MZF-1) transcription factor to the human GAPDH promoter. Furthermore, we show that up-regulation of MZF-1 by 1,25-dihydroxyvitamin D3 (calcitriol) induces GAPDH in HS-5 stromal fibroblasts, while knockdown of MZF1 by shRNA leads to a concomitant reduction in GAPDH expression. This argues that MZF-1 regulates GAPDH, indicating a role for GAPDH in calcitriol-mediated signaling.

Keywords: Calcitriol; GAPDH; Glycolysis; MZF1; Vitamin D.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Calcitriol / metabolism
  • Calcitriol / pharmacology*
  • Cell Line / drug effects
  • Fibroblasts / drug effects
  • Gene Expression Regulation / drug effects
  • Gene Knockdown Techniques
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism
  • Humans
  • Kruppel-Like Transcription Factors / genetics
  • Kruppel-Like Transcription Factors / metabolism*
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA, Small Interfering
  • Signal Transduction
  • Tretinoin / metabolism
  • Tretinoin / pharmacology

Substances

  • Kruppel-Like Transcription Factors
  • MZF1 protein, human
  • RNA, Small Interfering
  • Tretinoin
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Calcitriol