Sortase-based bio-organic strategies for macromolecular synthesis

V Haridas; Sandhya Sadanandan; N U Dheepthi

doi:10.1002/cbic.201402013

Sortase-based bio-organic strategies for macromolecular synthesis

Chembiochem. 2014 Sep 5;15(13):1857-67. doi: 10.1002/cbic.201402013. Epub 2014 Aug 8.

Authors

V Haridas¹, Sandhya Sadanandan, N U Dheepthi

Affiliation

¹ Department of Chemistry, Indian Institute of Technology Delhi, New Delhi 110016 (India). haridasv@iitd.ac.in.

PMID: 25111709
DOI: 10.1002/cbic.201402013

Abstract

Protein ligation sorted: SrtA is one of the molecules nature uses to perform chemoselective ligation on amazingly complex protein molecules. Sortase-mediated ligation (SML) with chemoselective reactions will find a variety of applications in chemistry, biology, and medicine in the near future.

Keywords: click chemistry; ligation; peptides; protein⋅thioester; sortase.

Publication types

Review

MeSH terms

Aminoacyltransferases / chemistry*
Bacterial Proteins / chemistry*
Carbohydrate Sequence
Cysteine Endopeptidases / chemistry*
Macromolecular Substances / chemical synthesis*
Molecular Sequence Data
Peptide Synthases / chemistry
Staphylococcus aureus / enzymology
Subtilisins / chemistry

Substances

Bacterial Proteins
Macromolecular Substances
Aminoacyltransferases
sortase A
Subtilisins
Cysteine Endopeptidases
Peptide Synthases
subtiligase