Methylcobalamin--a form of vitamin B12 identified and characterised in Chlorella vulgaris

Anantharajappa Kumudha; Sagaya Selvakumar; Pullancheri Dilshad; Gopal Vaidyanathan; Munna Singh Thakur; Ravi Sarada

doi:10.1016/j.foodchem.2014.08.035

Methylcobalamin--a form of vitamin B12 identified and characterised in Chlorella vulgaris

Food Chem. 2015 Mar 1:170:316-20. doi: 10.1016/j.foodchem.2014.08.035. Epub 2014 Aug 23.

Authors

Anantharajappa Kumudha¹, Sagaya Selvakumar², Pullancheri Dilshad³, Gopal Vaidyanathan³, Munna Singh Thakur², Ravi Sarada⁴

Affiliations

¹ Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
² Fermentation Technology and Bioengineering Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
³ Waters (India) Pvt. Ltd, Bangalore, India.
⁴ Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India. Electronic address: sarada_ravi@yahoo.com.

PMID: 25306351
DOI: 10.1016/j.foodchem.2014.08.035

Abstract

Vitamin B12 is among the most essential biomolecules required for crucial metabolic processes in humans. Vitamin B12 was extracted from Chlorella vulgaris biomass under aqueous conditions, partially purified by passing the extract through amberlite XAD-2, Sep-Pak columns, and further purified by HPLC. The target peak eluent was subjected to characterisation by tandem mass spectrometry (MS/MS), selected ion recording (SIR) and multiple reaction monitoring (MRM) and identified as methylcobalamin (Me-Cbl). Quantification of Me-Cbl was carried out by microbiological and chemiluminescence methods, and found to be 29.87±2 μg/100 g and 26.84±2 μg/100 g dry weight, respectively. The presence of Me-Cbl was further substantiated using gold nanoparticle (AuNPs) based aptamer analysis, and found to be 28.02±2 μg/100 g dry weight. Good similarity was observed among all the methods. Methylcobalamin, a form of vitamin B12 was identified in C. vulgaris and this finding enhances its use as a nutritional supplement.

Keywords: Aptamer; Chlorella; Gold nanoparticle; MS/MS; Methylcobalamin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorella vulgaris / chemistry*
Chromatography, High Pressure Liquid / methods*
Gold / analysis*
Humans
Nanoparticles / analysis*
Tandem Mass Spectrometry
Vitamin B 12 / analogs & derivatives*
Vitamin B 12 / analysis
Vitamin B 12 / chemistry*

Substances

Gold
mecobalamin
Vitamin B 12