Anosmia is a model to study the interaction among chemoreception systems. In the head injury, the traumatic irreversible anosmia caused by damage to olfactory nerve fibers and brain regions is of enviable research interest. In this study, psychophysiological tests for a comprehensive assessment of olfactory function were utilized to investigate anosmia, together with a new technique based on the breath real-time monitoring of volatile organic compounds (VOCs). We applied the breath and VOCs analysis to investigate chemoresponsiveness in the long-term irreversible post-traumatic anosmia.