Objective: To develop a highly sensitive method for the determination of platelet-rich plasma serotonin concentration for every-day clinical practice.
Material and methods: The method was developed for determination of serotonin in plateled-rich plasma. Serotonin was determined using high performance liquid chromatography with fluorescent detection and hematologic analyzer.
Results and conclusion: In healthy people, concentrations of platelet serotonin were similar to those reported in literature: from 2.19 to 4.87 nmol/109 platelets. In patients with depression (n=30), platelet serotonin concentrations were lower (from 0.11 to 2.89 nmol/109 platelets) and correlated with the severity of depression. The method may be used in clinical purposes.