Abstract
We describe a syncytia assay that utilizes a noninfectious plasmid (pEVd1443) derived from human immunodeficiency virus type I (HIV-I). This plasmid carries a large deletion of gag-pol sequences but expresses HIV-I envelope proteins and induces syncytia following transfection into HeLa-CD4 cells. This plasmid was used to study the effects of mutations in the gp 120 and gp41 portions of the envelope gene of HIV-I.
MeSH terms
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Antigens, Differentiation, T-Lymphocyte / genetics
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Blotting, Western
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Gene Expression Regulation
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Gene Products, gag
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Gene Products, tat
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HIV Antigens / genetics
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HIV Envelope Protein gp120
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HIV Envelope Protein gp160
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HIV Envelope Protein gp41
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HIV-1 / genetics*
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HIV-1 / immunology
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HeLa Cells
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Humans
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Mutation
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Plasmids
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Precipitin Tests
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Retroviridae Proteins / analysis*
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Retroviridae Proteins / genetics
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Transcription Factors / genetics
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Transfection
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Viral Envelope Proteins / analysis*
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Viral Envelope Proteins / genetics
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tat Gene Products, Human Immunodeficiency Virus
Substances
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Antigens, Differentiation, T-Lymphocyte
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Gene Products, gag
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Gene Products, tat
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HIV Antigens
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HIV Envelope Protein gp120
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HIV Envelope Protein gp160
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HIV Envelope Protein gp41
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Retroviridae Proteins
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Transcription Factors
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Viral Envelope Proteins
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tat Gene Products, Human Immunodeficiency Virus