A syncytia assay for human immunodeficiency virus type I (HIV-I) envelope protein and its use in studying HIV-I mutations

Virology. 1989 Jun;170(2):566-70. doi: 10.1016/0042-6822(89)90448-0.

Abstract

We describe a syncytia assay that utilizes a noninfectious plasmid (pEVd1443) derived from human immunodeficiency virus type I (HIV-I). This plasmid carries a large deletion of gag-pol sequences but expresses HIV-I envelope proteins and induces syncytia following transfection into HeLa-CD4 cells. This plasmid was used to study the effects of mutations in the gp 120 and gp41 portions of the envelope gene of HIV-I.

MeSH terms

  • Antigens, Differentiation, T-Lymphocyte / genetics
  • Blotting, Western
  • Gene Expression Regulation
  • Gene Products, gag
  • Gene Products, tat
  • HIV Antigens / genetics
  • HIV Envelope Protein gp120
  • HIV Envelope Protein gp160
  • HIV Envelope Protein gp41
  • HIV-1 / genetics*
  • HIV-1 / immunology
  • HeLa Cells
  • Humans
  • Mutation
  • Plasmids
  • Precipitin Tests
  • Retroviridae Proteins / analysis*
  • Retroviridae Proteins / genetics
  • Transcription Factors / genetics
  • Transfection
  • Viral Envelope Proteins / analysis*
  • Viral Envelope Proteins / genetics
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • Gene Products, gag
  • Gene Products, tat
  • HIV Antigens
  • HIV Envelope Protein gp120
  • HIV Envelope Protein gp160
  • HIV Envelope Protein gp41
  • Retroviridae Proteins
  • Transcription Factors
  • Viral Envelope Proteins
  • tat Gene Products, Human Immunodeficiency Virus