It has recently been suggested that sterility or inviability in species hybrids might result from the movement of transposable elements. Because such movement is often detectable by an increased mutation rate, I studied the effect of interspecific hybridization in the Drosophila melanogaster group on the mutation rate of X-linked visibles. This rate did not differ between hybrids and intraspecific controls. This was also true for the germ-line excision rate of a transposable element, although the rate of somatic excision was two to sixfold higher in hybrids than in pure species. Combined with previous work, these results do not support a role for transposable elements in speciation.