RNA Interference in Caenorhabditis elegans

Darryl Conte Jr; Lesley T MacNeil; Albertha J M Walhout; Craig C Mello

doi:10.1002/0471142727.mb2603s109

RNA Interference in Caenorhabditis elegans

Curr Protoc Mol Biol. 2015 Jan 5:109:26.3.1-26.3.30. doi: 10.1002/0471142727.mb2603s109.

Authors

Darryl Conte Jr¹, Lesley T MacNeil², Albertha J M Walhout², Craig C Mello^{1

3}

Affiliations

¹ RNA Therapeutics Institute and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.
² Programs in Systems Biology and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.
³ Howard Hughes Medical Institute, Chevy Chase, Maryland.

Abstract

RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens.

Keywords: C. elegans; RNAi; feeding; high-throughput screening assays; microinjection; soaking.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Caenorhabditis elegans / genetics*
Genetic Testing / methods
Molecular Biology / methods*
Parasitology / methods*
RNA Interference*

Abstract

Publication types

MeSH terms

Grants and funding