Generating iPSCs: translating cell reprogramming science into scalable and robust biomanufacturing strategies

Marli Silva; Laurence Daheron; Hannah Hurley; Kim Bure; Richard Barker; Andrew J Carr; David Williams; Hae-Won Kim; Anna French; Pete J Coffey; Justin J Cooper-White; Brock Reeve; Mahendra Rao; Evan Y Snyder; Kelvin S Ng; Benjamin E Mead; James A Smith; Jeffrey M Karp; David A Brindley; Ivan Wall

doi:10.1016/j.stem.2014.12.013

Generating iPSCs: translating cell reprogramming science into scalable and robust biomanufacturing strategies

Cell Stem Cell. 2015 Jan 8;16(1):13-7. doi: 10.1016/j.stem.2014.12.013.

Authors

Marli Silva¹, Laurence Daheron², Hannah Hurley³, Kim Bure⁴, Richard Barker³, Andrew J Carr⁵, David Williams⁶, Hae-Won Kim⁷, Anna French¹, Pete J Coffey⁸, Justin J Cooper-White⁹, Brock Reeve², Mahendra Rao¹⁰, Evan Y Snyder¹¹, Kelvin S Ng¹², Benjamin E Mead¹², James A Smith¹³, Jeffrey M Karp¹², David A Brindley¹⁴, Ivan Wall¹⁵

Affiliations

¹ Department of Biochemical Engineering, University College London, London, WC1H 0AH, UK.
² Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
³ The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK.
⁴ TAP Biosystems, Royston, Hertfordshire, SG8 5WY, UK.
⁵ The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK; Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Nuffield Orthopaedic Centre, University of Oxford, Oxford, OX3 7LD, UK.
⁶ Centre for Biological Engineering, Wolfson School of Mechanical and Manufacturing Engineering, Loughborough University, Loughborough, LE11 3TU, UK.
⁷ Department of Nanobiomedical Science and BK21 Plus NBM Global Research Center of Regenerative Medicine, Dankook University, Cheonan 330-714, Republic of Korea; Institute of Tissue Regeneration Engineering, Dankook University Graduate School, Cheonan 330-714, Republic of Korea; Department of Dental Biomaterials, School of Dentistry, Dankook University, Shinbu-dong, Cheonan 330-714, Republic of Korea.
⁸ Ocular Biology and Therapeutics, Institute of Ophthalmology, University College London, London, EC1V 9EL, UK; Neuroscience Research Institute, University of California, Santa Barbara, CA 93106-5060, USA.
⁹ Australian Institute for Bioengineering & Nanotechnology, The University of Queensland, St. Lucia, QLD 4072, Australia; School of Chemical Engineering, The University of Queensland, St. Lucia, QLD 4072, Australia; Materials Science and Engineering Division, CSIRO, Clayton, VIC 3169, Australia.
¹⁰ New York Stem Cell Foundation, New York, NY 10023, USA.
¹¹ Burnham Medical Research Institute, La Jolla, CA 92037, USA; Department of Pediatrics, University of California, San Diego, La Jolla, CA 92161, USA; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.
¹² Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
¹³ Harvard Stem Cell Institute, Cambridge, MA 02138, USA; The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK.
¹⁴ Harvard Stem Cell Institute, Cambridge, MA 02138, USA; The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK; Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Nuffield Orthopaedic Centre, University of Oxford, Oxford, OX3 7LD, UK; Centre for Behavioural Medicine, UCL School of Pharmacy, University College London, London WC1H 9JP, UK.
¹⁵ Department of Biochemical Engineering, University College London, London, WC1H 0AH, UK; Department of Nanobiomedical Science and BK21 Plus NBM Global Research Center of Regenerative Medicine, Dankook University, Cheonan 330-714, Republic of Korea; Institute of Tissue Regeneration Engineering, Dankook University Graduate School, Cheonan 330-714, Republic of Korea. Electronic address: i.wall@ucl.ac.uk.

PMID: 25575079
DOI: 10.1016/j.stem.2014.12.013

Abstract

Induced pluripotent stem cells (iPSCs) have the potential to transform drug discovery and healthcare in the 21(st) century. However, successful commercialization will require standardized manufacturing platforms. Here we highlight the need to define standardized practices for iPSC generation and processing and discuss current challenges to the robust manufacture of iPSC products.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods*
Cellular Reprogramming*
Humans
Induced Pluripotent Stem Cells / cytology*
Reproducibility of Results
Stem Cell Transplantation

Abstract

Publication types

MeSH terms

Grants and funding