Targeted gene disruption in Xenopus laevis using CRISPR/Cas9

Fengqin Wang; Zhaoying Shi; Yan Cui; Xiaogang Guo; Yun-Bo Shi; Yonglong Chen

doi:10.1186/s13578-015-0006-1

Targeted gene disruption in Xenopus laevis using CRISPR/Cas9

Cell Biosci. 2015 Apr 14:5:15. doi: 10.1186/s13578-015-0006-1. eCollection 2015.

Authors

Fengqin Wang¹, Zhaoying Shi², Yan Cui³, Xiaogang Guo⁴, Yun-Bo Shi⁵, Yonglong Chen⁶

Affiliations

¹ School of Life Sciences, Anhui University, Hefei, 230601 China ; CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.
² Department of Biology, Shenzhen Key Laboratory of Cell Microenvironment, South University of Science and Technology of China, Shenzhen, 518055 China.
³ CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China ; University of Chinese Academy of Sciences, Beijing, 100049 China.
⁴ CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.
⁵ Section on Molecular Morphogenesis, Program in Cellular Regulation and Metabolism, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), U.S. National Institutes of Health, Bethesda, Maryland USA.
⁶ Department of Biology, Shenzhen Key Laboratory of Cell Microenvironment, South University of Science and Technology of China, Shenzhen, 518055 China ; CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China.

No abstract available