Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells. Primary structure and functional expression from cDNAs

S Yokoyama; K Imoto; T Kawamura; H Higashida; N Iwabe; T Miyata; S Numa

doi:10.1016/0014-5793(89)81488-7

Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells. Primary structure and functional expression from cDNAs

FEBS Lett. 1989 Dec 18;259(1):37-42. doi: 10.1016/0014-5793(89)81488-7.

Authors

S Yokoyama¹, K Imoto, T Kawamura, H Higashida, N Iwabe, T Miyata, S Numa

Affiliation

¹ Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.

PMID: 2599109
DOI: 10.1016/0014-5793(89)81488-7

Abstract

The complete amino acid sequences of two potassium channel proteins from NG108-15 neuroblastoma-glioma hybrid cells have been deduced by cloning and sequencing the cDNAs. One of these proteins (NGK2) is structurally more closely related to the Drosophila Shaw gene product than to the Shaker and Shab gene products, whereas the other (NGK1) is identical with a rat brain potassium channel protein (BK2) which is more closely related to the Drosophila Shaker gene product. mRNAs derived from both the cloned cDNAs, when injected into Xenopus oocytes, direct the formation of functional potassium channels with properties of delayed rectifiers.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA / genetics
Mice
Molecular Sequence Data
Phylogeny
Potassium Channels / physiology*
Rats
Xenopus laevis

Substances

Potassium Channels
DNA

Associated data

GENBANK/Y07521