Fe65 Suppresses Breast Cancer Cell Migration and Invasion through Tip60 Mediated Cortactin Acetylation

Sci Rep. 2015 Jul 13:5:11529. doi: 10.1038/srep11529.

Abstract

Fe65 is a brain-enriched adaptor protein known for its role in the action of the Aβ amyloid precursor protein in neuronal cells and Alzheimer's disease, but little is known about its functions in cancer cells. The present study documents for the first time a role of Fe65 in suppressing breast cancer cell migration and invasion. Mechanistic studies suggest that the suppression is mediated through its phosphotyrosine binding domain 1 that mediates the recruitment of Tip60 to cortactin to stimulate its acetylation. The studies identify the Tip60 acetyltransferase as a cytoplasmic drug target for the therapeutic intervention of metastatic breast cancers.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetylation
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Cell Movement
  • Cortactin / metabolism*
  • Female
  • HEK293 Cells
  • Histone Acetyltransferases / metabolism*
  • Histone Deacetylase 6
  • Histone Deacetylases / metabolism
  • Humans
  • Immunoprecipitation
  • Lysine Acetyltransferase 5
  • Nerve Tissue Proteins / antagonists & inhibitors
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Nuclear Proteins / antagonists & inhibitors
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Protein Structure, Tertiary
  • RNA Interference
  • RNA, Small Interfering / metabolism

Substances

  • APBB1 protein, human
  • Cortactin
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • RNA, Small Interfering
  • Histone Acetyltransferases
  • KAT5 protein, human
  • Lysine Acetyltransferase 5
  • HDAC6 protein, human
  • Histone Deacetylase 6
  • Histone Deacetylases