Wide Dispersion and Diversity of Clonally Related Inhibitory Interneurons

Neuron. 2015 Sep 2;87(5):999-1007. doi: 10.1016/j.neuron.2015.07.030. Epub 2015 Aug 20.

Abstract

The mammalian neocortex is composed of two major neuronal cell types with distinct origins: excitatory pyramidal neurons and inhibitory interneurons, generated in dorsal and ventral progenitor zones of the embryonic telencephalon, respectively. Thus, inhibitory neurons migrate relatively long distances to reach their destination in the developing forebrain. The role of lineage in the organization and circuitry of interneurons is still not well understood. Utilizing a combination of genetics, retroviral fate mapping, and lineage-specific retroviral barcode labeling, we find that clonally related interneurons can be widely dispersed while unrelated interneurons can be closely clustered. These data suggest that migratory mechanisms related to the clustering of interneurons occur largely independent of their clonal origin.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Lineage
  • Cell Movement / physiology*
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Embryo, Mammalian
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • In Vitro Techniques
  • Interneurons / physiology*
  • Laser Capture Microdissection
  • Mice
  • Nerve Net / physiology*
  • Nerve Tissue Proteins / metabolism
  • Neural Inhibition*
  • Neural Stem Cells / classification
  • Neural Stem Cells / physiology*
  • Organ Culture Techniques
  • Telencephalon / cytology*
  • Telencephalon / embryology
  • Transduction, Genetic

Substances

  • Nerve Tissue Proteins
  • Green Fluorescent Proteins