We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing results for the prediction of inducible clarithromycin drug resistance.
Keywords: Drug susceptibility; Mycobacterium abscessus; Nontuberculous mycobacteria (NTM); Public health; Real-time PCR; Taqman; erm(41).
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