Rapid prediction of inducible clarithromycin resistance in Mycobacterium abscessus

Yan Chun Zhu; Kara K Mitchell; Elizabeth J Nazarian; Vincent E Escuyer; Kimberlee A Musser

doi:10.1016/j.mcp.2015.08.007

Rapid prediction of inducible clarithromycin resistance in Mycobacterium abscessus

Mol Cell Probes. 2015 Dec;29(6):514-516. doi: 10.1016/j.mcp.2015.08.007. Epub 2015 Sep 1.

Authors

Yan Chun Zhu¹, Kara K Mitchell¹, Elizabeth J Nazarian¹, Vincent E Escuyer¹, Kimberlee A Musser²

Affiliations

¹ Wadsworth Center, New York State Department of Health, Albany, NY, USA.
² Wadsworth Center, New York State Department of Health, Albany, NY, USA. Electronic address: kimberlee.musser@health.ny.gov.

PMID: 26334290
DOI: 10.1016/j.mcp.2015.08.007

Abstract

We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing results for the prediction of inducible clarithromycin drug resistance.

Keywords: Drug susceptibility; Mycobacterium abscessus; Nontuberculous mycobacteria (NTM); Public health; Real-time PCR; Taqman; erm(41).

MeSH terms

Bacterial Proteins / genetics*
Clarithromycin / pharmacology
Drug Resistance, Bacterial
Humans
Mycobacterium Infections, Nontuberculous / diagnosis
Mycobacterium Infections, Nontuberculous / microbiology
Nontuberculous Mycobacteria / classification
Nontuberculous Mycobacteria / drug effects
Nontuberculous Mycobacteria / genetics
Nontuberculous Mycobacteria / isolation & purification*
Real-Time Polymerase Chain Reaction / methods*

Substances

Bacterial Proteins
Clarithromycin