Rapid prediction of inducible clarithromycin resistance in Mycobacterium abscessus

Mol Cell Probes. 2015 Dec;29(6):514-516. doi: 10.1016/j.mcp.2015.08.007. Epub 2015 Sep 1.

Abstract

We have developed a single tube TaqMan(®) real-time PCR assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in Mycobacterium abscessus. A study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing results for the prediction of inducible clarithromycin drug resistance.

Keywords: Drug susceptibility; Mycobacterium abscessus; Nontuberculous mycobacteria (NTM); Public health; Real-time PCR; Taqman; erm(41).

MeSH terms

  • Bacterial Proteins / genetics*
  • Clarithromycin / pharmacology
  • Drug Resistance, Bacterial
  • Humans
  • Mycobacterium Infections, Nontuberculous / diagnosis
  • Mycobacterium Infections, Nontuberculous / microbiology
  • Nontuberculous Mycobacteria / classification
  • Nontuberculous Mycobacteria / drug effects
  • Nontuberculous Mycobacteria / genetics
  • Nontuberculous Mycobacteria / isolation & purification*
  • Real-Time Polymerase Chain Reaction / methods*

Substances

  • Bacterial Proteins
  • Clarithromycin