Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis

Nat Struct Mol Biol. 2015 Nov;22(11):920-3. doi: 10.1038/nsmb.3111. Epub 2015 Oct 19.

Abstract

Eukaryotic ribosome biogenesis involves a plethora of ribosome-assembly factors, and their temporal order of association with preribosomal RNA is largely unknown. By using Saccharomyces cerevisiae as a model organism, we developed a system that recapitulates and arrests ribosome assembly at early stages, thus providing in vivo snapshots of nascent preribosomal particles. Here we report the stage-specific order in which 70 ribosome-assembly factors associate with preribosomal RNA domains, thereby forming the 6-MDa small-subunit processome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Macromolecular Substances / metabolism
  • Models, Biological
  • Organelle Biogenesis*
  • RNA, Ribosomal / metabolism*
  • Ribosomal Proteins / metabolism*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / physiology*

Substances

  • Macromolecular Substances
  • RNA, Ribosomal
  • Ribosomal Proteins