Genome-wide RNA profiling of long-lasting stem cell-like memory CD8 T cells induced by Yellow Fever vaccination in humans

Silvia A Fuertes Marraco; Charlotte Soneson; Mauro Delorenzi; Daniel E Speiser

doi:10.1016/j.gdata.2015.06.024

Genome-wide RNA profiling of long-lasting stem cell-like memory CD8 T cells induced by Yellow Fever vaccination in humans

Genom Data. 2015 Jul 2:5:297-301. doi: 10.1016/j.gdata.2015.06.024. eCollection 2015 Sep.

Authors

Silvia A Fuertes Marraco¹, Charlotte Soneson², Mauro Delorenzi³, Daniel E Speiser¹

Affiliations

¹ Ludwig Cancer Center, University of Lausanne, Switzerland ; Department of Oncology, Lausanne University Hospital (CHUV), Switzerland.
² Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.
³ Ludwig Cancer Center, University of Lausanne, Switzerland ; Department of Oncology, Lausanne University Hospital (CHUV), Switzerland ; Bioinformatics Core Facility, SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.

Abstract

The live-attenuated Yellow Fever (YF) vaccine YF-17D induces a broad and polyfunctional CD8 T cell response in humans. Recently, we identified a population of stem cell-like memory CD8 T cells induced by YF-17D that persists at stable frequency for at least 25 years after vaccination. The YF-17D is thus a model system of human CD8 T cell biology that furthermore allows to track and study long-lasting and antigen-specific human memory CD8 T cells. Here, we describe in detail the sample characteristics and preparation of a microarray dataset acquired for genome-wide gene expression profiling of long-lasting YF-specific stem cell-like memory CD8 T cells, compared to the reference CD8 T cell differentiation subsets from total CD8 T cells. We also describe the quality controls, annotations and exploratory analyses of the dataset. The microarray data is available from the Gene Expression Omnibus (GEO) public repository with accession number GSE65804.