The role of protein oxidation and DNA damage in elderly hypertension

Aging Clin Exp Res. 2016 Aug;28(4):625-32. doi: 10.1007/s40520-015-0464-7. Epub 2015 Oct 20.

Abstract

Introduction: This study aimed to evaluate the role of protein oxidation and DNA damage in the elderly hypertensive (HT) patients.

Materials and methods: This study consisted of four groups: two elderly groups with 30 HT patients and 30 normotensive healthy volunteers, and two young groups with 30 HT patients and 30 normotensive healthy volunteers. Plasma total thiol (T-SH), advanced oxidation protein products (AOPPs), protein carbonyl (PCO), ischemia modified albumin (IMA), urine 8-hydroxy-2'-deoxyguanosine (8-OHdG), and prooxidant-antioxidant balance (PAB) levels were measured.

Results: In the elderly HT group AOPPs, PCO, 8-OHdG, and PAB were significantly higher than the elderly control group. In the young HT group T-SH levels were significantly lower and the other oxidative stress parameters were significantly higher than the young control group. In the elderly control group AOPPs, PCO, IMA, 8-OHdG and PAB were significantly higher than the young control group. T-SH was significantly lower in the elderly control than the young control group. In the elderly HT group, T-SH levels were significantly lower and AOPPs, PCO, IMA, 8-OHdG, and PAB levels were significantly higher than the young HT group.

Conclusion: Protein and DNA cell damage occurs by oxidation of free radicals throughout life. Our study supports the view that these radicals may be responsible for the development of hypertension with aging process. Urine 8-OHdG levels can be used as a marker for oxidative DNA damage in the elderly hypertensive patients. Finally, our results suggest that oxidative stress may influence both the development and progression of hypertension and aging.

Keywords: Aging; DNA damage; Hypertension; Oxidative stress; Protein oxidation.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Aged
  • Aged, 80 and over
  • Aging / physiology
  • Antioxidants / metabolism*
  • Biomarkers / metabolism
  • DNA Damage / physiology*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / urine
  • Female
  • Free Radicals / metabolism
  • Humans
  • Hypertension / physiopathology*
  • Male
  • Middle Aged
  • Oxidation-Reduction
  • Oxidative Stress*
  • Serum Albumin / metabolism
  • Serum Albumin, Human
  • Sulfhydryl Compounds / metabolism

Substances

  • Antioxidants
  • Biomarkers
  • Free Radicals
  • Serum Albumin
  • Sulfhydryl Compounds
  • ischemia-modified albumin
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine
  • Serum Albumin, Human