Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number

Mol Microbiol. 1989 Jul;3(7):903-10. doi: 10.1111/j.1365-2958.1989.tb00239.x.

Abstract

A gene, pcnB, affecting the copy number of ColE1-related plasmids has been cloned and mapped to 3.6 min on the Escherichia coli chromosome between panD and fhu. The gene encodes a previously undescribed 48 kD protein. Several independently isolated mutants exhibiting the same phenotype, reduced copy number, have been shown to be pcnB-.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Ampicillin Resistance
  • Bacterial Proteins / genetics*
  • Cloning, Molecular
  • Escherichia coli / genetics*
  • Gene Expression Regulation
  • Genes, Bacterial*
  • Molecular Weight
  • Mutation
  • Phenotype
  • Plasmids*
  • Protein Biosynthesis
  • Restriction Mapping
  • Transcription, Genetic
  • Transduction, Genetic

Substances

  • Bacterial Proteins