Evaluation of immunochromatographic tests for the rapid detection of the emerging GII.17 norovirus in stool samples, January 2016

Euro Surveill. 2016;21(4). doi: 10.2807/1560-7917.ES.2016.21.4.30115.

Abstract

A novel GII.17 norovirus emerged in Asia in the winter of 2014/15. A worldwide spread is conceivable and norovirus diagnostic assays need to be evaluated to investigate if they adequately detect this emerging genotype. Seven immunochromatographic kits commercially available in Europe were evaluated on ten stool samples where GII.17 virus had been quantified by real-time reverse transcription-polymerase chain reaction. All the kits detected GII.17 with various sensitivities, partly depending on the virus titre.

Keywords: Immunochromatograhic tests; Norovirus GII.17.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Antigens, Viral / analysis*
  • Asia
  • Caliciviridae Infections / diagnosis*
  • Caliciviridae Infections / virology
  • Child
  • Child, Preschool
  • Chromatography, Affinity / methods*
  • Clinical Laboratory Techniques / methods*
  • Feces / virology*
  • Female
  • Gastroenteritis / diagnosis
  • Gastroenteritis / virology*
  • Genotype
  • Humans
  • Infant
  • Male
  • Norovirus / classification
  • Norovirus / genetics
  • Norovirus / isolation & purification*
  • Reagent Kits, Diagnostic
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Sequence Analysis, RNA
  • Viral Load
  • Young Adult

Substances

  • Antigens, Viral
  • Reagent Kits, Diagnostic