Draft genome of Haloarcula rubripromontorii strain SL3, a novel halophilic archaeon isolated from the solar salterns of Cabo Rojo, Puerto Rico

Rubén Sánchez-Nieves; Marc Facciotti; Sofía Saavedra-Collado; Lizbeth Dávila-Santiago; Roy Rodríguez-Carrero; Rafael Montalvo-Rodríguez

doi:10.1016/j.gdata.2016.02.005

Draft genome of Haloarcula rubripromontorii strain SL3, a novel halophilic archaeon isolated from the solar salterns of Cabo Rojo, Puerto Rico

Genom Data. 2016 Feb 6:7:287-9. doi: 10.1016/j.gdata.2016.02.005. eCollection 2016 Mar.

Authors

Rubén Sánchez-Nieves¹, Marc Facciotti², Sofía Saavedra-Collado¹, Lizbeth Dávila-Santiago¹, Roy Rodríguez-Carrero¹, Rafael Montalvo-Rodríguez¹

Affiliations

¹ Biology Department, University of Puerto Rico, Mayaguez, Box 9000, 00681-9000, Puerto Rico.
² Biomedical Engineering and Genome Center, 451 Health Sciences Drive, Davis, CA, 95618, United States.

Abstract

The genus Haloarcula belongs to the family Halobacteriaceae which currently has 10 valid species. Here we report the draft genome sequence of strain SL3, a new species within this genus, isolated from the Solar Salterns of Cabo Rojo, Puerto Rico. Genome assembly performed using NGEN Assembler resulted in 18 contigs (N50 = 601,911 bp), the largest of which contains 1,023,775 bp. The genome consists of 3.97 MB and has a GC content of 61.97%. Like all species of Haloarcula, the genome encodes heterogeneous copies of the small subunit ribosomal RNA. In addition, the genome includes 6 rRNAs, 48 tRNAs, and 3797 protein coding sequences. Several carbohydrate-active enzymes genes were found, as well as enzymes involved in the dihydroxyacetone processing pathway which are not found in other Haloarcula species. The NCBI accession number for this genome is LIUF00000000 and the strain deposit number is CECT9001.

Keywords: Halophilic archaea; Henome sequence; Puerto Rico.