Identification of immunodiagnostic antigens for cerebrospinal filariasis in horses by western blot analysis

Masataka Takesue; Yuki Osaka; Masanori Muranaka; Yoshinari Katayama; Hiromi Ikadai

doi:10.1294/jes.27.17

Identification of immunodiagnostic antigens for cerebrospinal filariasis in horses by western blot analysis

J Equine Sci. 2016;27(1):17-8. doi: 10.1294/jes.27.17. Epub 2016 Apr 12.

Authors

Masataka Takesue¹, Yuki Osaka¹, Masanori Muranaka², Yoshinari Katayama², Hiromi Ikadai¹

Affiliations

¹ Laboratory of Veterinary Parasitology, School of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan.
² Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.

Abstract

In the present study, the serum and cerebrospinal fluid of horses diagnosed with Setaria digitata cerebrospinal filariasis were analyzed by western blot. The results revealed S. digitata protein bands measuring 65, 34, 22, and 18 kDa in molecular weight. In particular, the 18 kDa band is a possible candidate for clinical immunodiagnosis on the basis of western blot findings.

Keywords: Setaria digitata; cerebrospinal filariasis; western blot.