Eggs of 12 strains of Aedes albopictus (Skuse), two strains of A. aegypti L., and one strain of A. triseriatus (Say) were tested for ability to overwinter at three outdoor locations in Indiana. Some survival of A. albopictus eggs was observed during the winters of 1986-1987 and 1987-1988. A. triseriatus had greater overwintering ability than A. albopictus; A. aegypti did not survive at all locations. Prolonged cold-conditioning and photoperiodically induced diapause increased the overwintering ability of A. albopictus eggs. Strains of A. albopictus from northern Asia and North America showed higher overwintering survival rates than A. albopictus strains from tropical Asia, Hawaii, and Brazil; strains from Indiana had greater ability to overwinter than those from Texas, Louisiana, and Florida. Results are discussed in relation to the overwintering limits of this species in the United States.