A versatile plasmonic thermogel for disinfection of antimicrobial resistant bacteria

Mohamed A Abdou Mohamed; Vahid Raeesi; Patricia V Turner; Anu Rebbapragada; Kate Banks; Warren C W Chan

doi:10.1016/j.biomaterials.2016.04.009

A versatile plasmonic thermogel for disinfection of antimicrobial resistant bacteria

Biomaterials. 2016 Aug:97:154-63. doi: 10.1016/j.biomaterials.2016.04.009. Epub 2016 Apr 22.

Authors

Mohamed A Abdou Mohamed¹, Vahid Raeesi², Patricia V Turner³, Anu Rebbapragada⁴, Kate Banks⁵, Warren C W Chan⁶

Affiliations

¹ Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St. 407, Toronto, ON, Canada; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 164 College St. 407, Toronto, ON, Canada; Botany and Microbiology Department, Faculty of Science, Zagzig University, Zagazig, Egypt.
² Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 164 College St. 407, Toronto, ON, Canada; Materials Science and Engineering, University of Toronto, 164 College St. 407, Toronto, ON, Canada.
³ Department of Pathobiology, University of Guelph, Guelph, ON, Canada.
⁴ Gamma-Dynacare Medical Laboratories, Brampton, ON, Canada.
⁵ Division of Comparative Medicine, Faculty of Medicine, University of Toronto, Toronto, ON, Canada; Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
⁶ Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College St. 407, Toronto, ON, Canada; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 164 College St. 407, Toronto, ON, Canada; Chemistry, University of Toronto, 164 College St. 407, Toronto, ON, Canada; Chemical Engineering, University of Toronto, 164 College St. 407, Toronto, ON, Canada; Materials Science and Engineering, University of Toronto, 164 College St. 407, Toronto, ON, Canada. Electronic address: warren.chan@utoronto.ca.

PMID: 27174687
DOI: 10.1016/j.biomaterials.2016.04.009

Abstract

The increasing occurrence of antimicrobial resistance among bacteria is a global problem that requires the development of alternative techniques to eradicate these superbugs. Herein, we used a combination of thermosensitive biocompatible polymer and gold nanorods to specifically deliver, preserve and confine heat to the area of interest. Our data demonstrates that this technique can be used to kill both Gram positive and Gram negative antimicrobial resistant bacteria in vitro. Our approach significantly reduces the antimicrobial resistant bacteria load in experimentally infected wounds by 98% without harming the surrounding tissues. More importantly, this polymer-nanocomposite can be prepared easily and applied to the wounds, can generate heat using a hand-held laser device, is safe for the operator, and does not have any adverse effects on the wound tissue and healing process.

Keywords: Antibiotic resistant bacteria; Gold nanorods; Photothermal; Thermogel; Wound infections.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Infective Agents / pharmacology*
Bacteria / drug effects*
Caprolactam / chemistry
Disinfection*
Drug Resistance, Bacterial / drug effects*
Gels / chemistry*
Hyperthermia, Induced
Microbial Sensitivity Tests
Microbial Viability / drug effects
Nanotubes / chemistry
Phototherapy
Rats
Temperature*

Substances

Anti-Infective Agents
Gels
Caprolactam
capron

Abstract

Publication types

MeSH terms

Substances

Grants and funding