In vivo bioluminescence and reflectance imaging of multiple organs in bioluminescence reporter mice by bundled-fiber-coupled microscopy

Yoriko Ando; Takashi Sakurai; Kowa Koida; Hajime Tei; Akiko Hida; Kazuki Nakao; Mistuo Natsume; Rika Numano

doi:10.1364/BOE.7.000963

In vivo bioluminescence and reflectance imaging of multiple organs in bioluminescence reporter mice by bundled-fiber-coupled microscopy

Biomed Opt Express. 2016 Feb 23;7(3):963-78. doi: 10.1364/BOE.7.000963. eCollection 2016 Mar 1.

Authors

Yoriko Ando¹, Takashi Sakurai², Kowa Koida³, Hajime Tei⁴, Akiko Hida⁵, Kazuki Nakao⁶, Mistuo Natsume⁷, Rika Numano⁸

Affiliations

¹ Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan.
² Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan; Juntendo University, Bunkyo-ku, Tokyo, 113-8421, Japan.
³ Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan; Department of Computer Science and Engineering, Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan.
⁴ Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan.
⁵ Department of Psychophysiology, National Institute of Mental Health, National Center of Neurology and Psychiatry, Kodaira, Tokyo, 187-8553 Japan.
⁶ Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan; Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Kobe, Hyogo, 650-0047, Japan.
⁷ Denkosha Co., Ltd., Hamamatsu, Shizuoka, 432-8055, Japan.
⁸ Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan; Department of Environmental and Life Science, Biological Regulatory Engineering, Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan.

Abstract

Bioluminescence imaging (BLI) is used in biomedical research to monitor biological processes within living organisms. Recently, fiber bundles with high transmittance and density have been developed to detect low light with high resolution. Therefore, we have developed a bundled-fiber-coupled microscope with a highly sensitive cooled-CCD camera that enables the BLI of organs within the mouse body. This is the first report of in vivo BLI of the brain and multiple organs in luciferase-reporter mice using bundled-fiber optics. With reflectance imaging, the structures of blood vessels and organs can be seen clearly with light illumination, and it allowed identification of the structural details of bioluminescence images. This technique can also be applied to clinical diagnostics in a low invasive manner.

Keywords: (110.2350) Fiber optics imaging; (170.2150) Endoscopic imaging; (170.3880) Medical and biological imaging.