Structural role of the T94I rhodopsin mutation in congenital stationary night blindness

EMBO Rep. 2016 Oct;17(10):1431-1440. doi: 10.15252/embr.201642671. Epub 2016 Jul 25.

Abstract

Congenital stationary night blindness (CSNB) is an inherited and non-progressive retinal dysfunction. Here, we present the crystal structure of CSNB-causing T94I2.61 rhodopsin in the active conformation at 2.3 Å resolution. The introduced hydrophobic side chain prolongs the lifetime of the G protein activating metarhodopsin-II state by establishing a direct van der Waals contact with K2967.43, the site of retinal attachment. This is in stark contrast to the light-activated state of the CSNB-causing G90D2.57 mutation, where the charged mutation forms a salt bridge with K2967.43 To find the common denominator between these two functional modifications, we combined our structural data with a kinetic biochemical analysis and molecular dynamics simulations. Our results indicate that both the charged G90D2.57 and the hydrophobic T94I2.61 mutation alter the dark state by weakening the interaction between the Schiff base (SB) and its counterion E1133.28 We propose that this interference with the tight regulation of the dim light photoreceptor rhodopsin increases background noise in the visual system and causes the loss of night vision characteristic for CSNB patients.

Keywords: G protein‐coupled receptors; congenital stationary night blindness; constitutive activity; rhodopsin; visual system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Catalytic Domain
  • Darkness
  • Eye Diseases, Hereditary / genetics*
  • Genetic Association Studies
  • Genetic Diseases, X-Linked / genetics*
  • Humans
  • Models, Biological
  • Models, Molecular
  • Mutation*
  • Myopia / genetics*
  • Night Blindness / genetics*
  • Protein Binding
  • Protein Conformation
  • Protein Stability
  • Rhodopsin / chemistry*
  • Rhodopsin / genetics*
  • Schiff Bases / chemistry
  • Structure-Activity Relationship
  • Thermodynamics

Substances

  • Schiff Bases
  • Rhodopsin

Supplementary concepts

  • Night blindness, congenital stationary