Effect of Circulation Parameters on Functional Status of HepaRG Spheroids Cultured in Microbioreactor

O V Semenova; V A Petrov; T N Gerasimenko; A V Aleksandrova; O A Burmistrova; A A Khutornenko; A I Osipyants; A A Poloznikov; D A Sakharov

doi:10.1007/s10517-016-3430-2

Effect of Circulation Parameters on Functional Status of HepaRG Spheroids Cultured in Microbioreactor

Bull Exp Biol Med. 2016 Jul;161(3):425-9. doi: 10.1007/s10517-016-3430-2. Epub 2016 Aug 6.

Authors

O V Semenova¹, V A Petrov¹, T N Gerasimenko¹, A V Aleksandrova¹, O A Burmistrova¹, A A Khutornenko², A I Osipyants¹, A A Poloznikov¹, D A Sakharov¹

Affiliations

¹ Moscow Aircraft Construction Institute (National Research University), Moscow, Russia.
² Moscow Aircraft Construction Institute (National Research University), Moscow, Russia. n.khutornenko@bioclinicum.com.

PMID: 27496037
DOI: 10.1007/s10517-016-3430-2

Abstract

We studied the relationship between microcirculation parameters and functional status of HepaRG cells in spheroids and chose an optimal regimen within the physiologically permissible limits of mechanical impact for the cells that maintains the expression of functional genes of the liver.

Keywords: HepaRG spheroids; liver-on-a-chip; microcirculation.

MeSH terms

Bioreactors
Cell Survival / physiology
Hepatocytes / cytology
Humans
Liver / cytology*
Microcirculation / physiology