Functional engineered human cardiac patches prepared from nature's platform improve heart function after acute myocardial infarction

Qingjie Wang; Hui Yang; Aobing Bai; Wei Jiang; Xiuya Li; Xinhong Wang; Yishen Mao; Chao Lu; Ruizhe Qian; Feng Guo; Tianling Ding; Haiyan Chen; Sifeng Chen; Jianyi Zhang; Chen Liu; Ning Sun

doi:10.1016/j.biomaterials.2016.07.035

Functional engineered human cardiac patches prepared from nature's platform improve heart function after acute myocardial infarction

Biomaterials. 2016 Oct:105:52-65. doi: 10.1016/j.biomaterials.2016.07.035. Epub 2016 Jul 29.

Authors

Qingjie Wang¹, Hui Yang¹, Aobing Bai¹, Wei Jiang¹, Xiuya Li¹, Xinhong Wang¹, Yishen Mao¹, Chao Lu¹, Ruizhe Qian¹, Feng Guo², Tianling Ding³, Haiyan Chen⁴, Sifeng Chen¹, Jianyi Zhang⁵, Chen Liu⁶, Ning Sun⁷

Affiliations

¹ Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
² Department of Cell Biology and Genetics, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
³ Department of Hematology, Huashan Hospital, Fudan University, Shanghai, 200032, China.
⁴ Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
⁵ Department of Biomedical Engineering, University of Alabama, Birmingham, AL, 35294, USA.
⁶ Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. Electronic address: liu.chen@zs-hospital.sh.cn.
⁷ Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China; Research Center on Aging and Medicine, Fudan University, Shanghai, 200032, China; State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200032, China. Electronic address: sunning@fudan.edu.cn.

PMID: 27509303
DOI: 10.1016/j.biomaterials.2016.07.035

Abstract

With the advent of induced pluripotent stem cells and directed differentiation techniques, it is now feasible to derive individual-specific cardiac cells for human heart tissue engineering. Here we report the generation of functional engineered human cardiac patches using human induced pluripotent stem cells-derived cardiac cells and decellularized natural heart ECM as scaffolds. The engineered human cardiac patches can be tailored to any desired size and shape and exhibited normal contractile and electrical physiology in vitro. Further, when patching on the infarct area, these patches improved heart function of rats with acute myocardial infarction in vivo. These engineered human cardiac patches can be of great value for normal and disease-specific heart tissue engineering, drug screening, and meet the demands for individual-specific heart tissues for personalized regenerative therapy of myocardial damages in the future.

Keywords: Cardiac patch; Cardiomyocytes; Decellularized heart matrix; Engineering human heart tissue; Induced pluripotent stem cells; Myocardial infarction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Disease
Animals
Cell Differentiation
Cell Proliferation
Cell-Free System
Extracellular Matrix / chemistry*
Fibroblasts / cytology
Fibroblasts / transplantation*
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / transplantation*
Male
Myocardial Infarction / pathology
Myocardial Infarction / therapy*
Myocytes, Cardiac / cytology*
Myocytes, Cardiac / transplantation
Organ Culture Techniques / instrumentation
Organ Culture Techniques / methods
Rats
Rats, Sprague-Dawley
Tissue Engineering / instrumentation
Tissue Engineering / methods*
Tissue Scaffolds*
Treatment Outcome

Grants and funding

U01 HL100407/HL/NHLBI NIH HHS/United States