Abstract
Bacteroides vulgatus is a member of the human microbiota whose abundance is increased in patients with Crohn's disease. We show that a B. vulgatus glycoside hydrolase from the carbohydrate active enzyme family GH123, BvGH123, is an N-acetyl-β-galactosaminidase that acts with retention of stereochemistry, and, through a 3-D structure in complex with Gal-thiazoline, provide evidence in support of a neighbouring group participation mechanism.
MeSH terms
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Acetylglucosamine / analogs & derivatives
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Acetylglucosamine / chemistry
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Acetylglucosamine / metabolism
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Bacteroides / enzymology*
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Binding Sites
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Catalytic Domain
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Crohn Disease / etiology
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Crohn Disease / microbiology
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Humans
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Molecular Dynamics Simulation
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Stereoisomerism
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Substrate Specificity
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Thiazoles / chemistry
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Thiazoles / metabolism
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beta-N-Acetyl-Galactosaminidase / chemistry
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beta-N-Acetyl-Galactosaminidase / metabolism*
Substances
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Thiazoles
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4-nitrophenyl-N-acetyl-2-deoxyglucopyranoside
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beta-N-Acetyl-Galactosaminidase
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Acetylglucosamine