Protective effects of oridonin on the sepsis in mice

Kaohsiung J Med Sci. 2016 Sep;32(9):452-7. doi: 10.1016/j.kjms.2016.07.013. Epub 2016 Aug 25.

Abstract

This study aimed to investigate the protective effects of oridonin (ORI) on cecal ligation and puncture (CLP)-induced sepsis in mice. Male C57BL/6 mice weighing 22-30 g and aged 8-10 weeks were randomly assigned to three groups: Sham group, CLP group, or CLP plus ORI group. In the CLP group and ORI group, CLP was induced, and intraperitoneal injection of normal saline and oridonin (100 μg/kg) was conducted, respectively. The survival rate was determined within the following 7 days. The blood, liver, and lung were collected at 24 hours after injury. Hematoxylin-eosin staining of the lung, detection of lung wet-to-dry ratio, and serum cytokines (tumor necrosis factor [TNF]-α and interleukin [IL]-6), and examination of intraperitoneal and blood bacterial clearance were conducted to evaluate the therapeutic efficacy. Results showed that ORI treatment significantly reduced the lung wet-to-dry ratio, decreased serum TNF-α and IL-6, and improved liver pathology compared with the CLP group (p < 0.05). Moreover, the intraperitoneal and blood bacterial clearance increased markedly after ORI treatment (p < 0.05). The 7-day survival rate in the ORI group was also dramatically higher than in the CLP group (p < 0.05). Our findings indicate that ORI can attenuate liver and lung injuries and elevate bacterial clearance to increase the survival rate of sepsis mice.

Keywords: Cecal ligation and puncture; Mouse; Oridonin; Proinflammatory cytokine; Sepsis.

MeSH terms

  • Animals
  • Colony Count, Microbial
  • Diterpenes, Kaurane / pharmacology
  • Diterpenes, Kaurane / therapeutic use*
  • Interleukin-6 / blood
  • Liver / drug effects
  • Liver / pathology
  • Lung / drug effects
  • Lung / pathology
  • Male
  • Mice, Inbred C57BL
  • Organ Size / drug effects
  • Peritoneum / drug effects
  • Peritoneum / microbiology
  • Peritoneum / pathology
  • Protective Agents / pharmacology
  • Protective Agents / therapeutic use*
  • Sepsis / blood
  • Sepsis / drug therapy*
  • Sepsis / pathology
  • Survival Analysis
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Diterpenes, Kaurane
  • Interleukin-6
  • Protective Agents
  • Tumor Necrosis Factor-alpha
  • oridonin