Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007

Bin-Yan Wu; Jian-Ren Ye; Lin Huang; Ling-Min He; De-Wei Li

doi:10.1016/j.mimet.2016.10.004

Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007

J Microbiol Methods. 2017 Jan:132:95-98. doi: 10.1016/j.mimet.2016.10.004. Epub 2016 Oct 8.

Authors

Bin-Yan Wu¹, Jian-Ren Ye², Lin Huang¹, Ling-Min He¹, De-Wei Li³

Affiliations

¹ College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China.
² College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China. Electronic address: jrye@njfu.edu.cn.
³ College of Forestry and Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forestry University, Nanjing, Jiangsu, China; The Connecticut Agricultural Experiment Station, Valley Laboratory, 153 Cook Hill Road, Windsor, CT, United States.

PMID: 27725176
DOI: 10.1016/j.mimet.2016.10.004

Abstract

Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.

Keywords: Burkholderia pyrrocinia; Expression stability; RT-qPCR; Reference genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Burkholderia / classification
Burkholderia / genetics*
Burkholderia / isolation & purification*
Genes, Bacterial*
Real-Time Polymerase Chain Reaction*

Substances

Bacterial Proteins