SnapShot: Class 2 CRISPR-Cas Systems

Kira S Makarova; Feng Zhang; Eugene V Koonin

doi:10.1016/j.cell.2016.12.038

SnapShot: Class 2 CRISPR-Cas Systems

Cell. 2017 Jan 12;168(1-2):328-328.e1. doi: 10.1016/j.cell.2016.12.038. Epub 2017 Jan 12.

Authors

Kira S Makarova¹, Feng Zhang², Eugene V Koonin¹

Affiliations

¹ National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA.
² Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

PMID: 28086097
DOI: 10.1016/j.cell.2016.12.038

Abstract

Class 2 CRISPR-Cas systems are characterized by effector modules consisting of single, large, multidomain proteins that appear to have been derived from mobile genetic elements. Some Class 2 effector proteins, such as Cas9 and Cas12a (Cpf1), have been successfully repurposed for genome engineering.

Published by Elsevier Inc.

MeSH terms

Bacteria / classification
Bacteria / genetics
Bacteria / immunology*
CRISPR-Cas Systems*
Endonucleases / chemistry
Endonucleases / genetics
Gene Editing

Substances

Endonucleases