The ciliated protozoan Vorticella convallaria is noted for its exceptionally fast adenosine triphosphate-independent cellular contraction, but direct measurements of contractile force have proven difficult given the length scale, speed, and forces involved. We used high-speed video microscopy to image live Vorticella stalled in midcontraction by deflection of an attached micropipette. Stall forces correlate with both distance contracted and the resting stalk length. Estimated isometric forces range from 95 to 177 nanonewtons (nN), or 1.12 nN·μm-1 of the stalk. Maximum velocity and work are also proportional to distance contracted. These parameters constrain proposed biochemical/physical models of the contractile mechanism.
Keywords: Vorticella; biomimicry; ciliate; molecular spring; motility; stall force.
© Published 2017. This article is a U.S. Government work and is in the public domain in the USA.