Biomanufacturing Seamless Tubular and Hollow Collagen Scaffolds with Unique Design Features and Biomechanical Properties

Anirudha Singh; David Lee; Nikolai Sopko; Hotaka Matsui; Praveena Sabnekar; Xiaopu Liu; Jennifer Elisseeff; Mark P Schoenberg; Kenneth Pienta; Trinity J Bivalacqua

doi:10.1002/adhm.201601136

Biomanufacturing Seamless Tubular and Hollow Collagen Scaffolds with Unique Design Features and Biomechanical Properties

Adv Healthc Mater. 2017 Mar;6(5). doi: 10.1002/adhm.201601136. Epub 2017 Jan 30.

Authors

Anirudha Singh^{1

2

3}, David Lee³, Nikolai Sopko¹, Hotaka Matsui¹, Praveena Sabnekar¹, Xiaopu Liu¹, Jennifer Elisseeff³, Mark P Schoenberg⁴, Kenneth Pienta^{1

5}, Trinity J Bivalacqua^{1

5}

Affiliations

¹ Department of Urology, The James Buchanan Brady Urological Institute, The Johns Hopkins School of Medicine, Baltimore, MD, 21287, USA.
² Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
³ Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD, 21231, USA.
⁴ Department of Urology, Albert Einstein College of Medicine, Bronx, NY, 10467, USA.
⁵ Department of Surgery and Oncology, Johns Hopkins Medical Institutions and Sidney Kimmel Comprehensive Cancer Center (SKCC), Baltimore, MD, 21287, USA.

PMID: 28135047
DOI: 10.1002/adhm.201601136

Abstract

A versatile process to develop designer collagen scaffolds for hollow and tubular tissue engineering applications is presented. This process creates seamless and biomechanically tunable scaffolds ranging from ureter-like microsized tubings to structures with highly customized lumens that resemble intestinal villi, fluid bladders, and alveolar sacs that together with stem cells can potentially be used in preclinical and clinical settings.

Keywords: biomanufacturing; collagen; neo-urinary bladders; neo-urinary conduits; scaffolds.

MeSH terms

Bioprosthesis*
Collagen / chemistry*
Humans
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / metabolism*
Tissue Scaffolds / chemistry*

Substances

Collagen