Detection and purification of bovine herpesvirus 1 glycoproteins by lectin affinity

J Virol Methods. 1988 Oct;22(1):23-9. doi: 10.1016/0166-0934(88)90084-5.

Abstract

A procedure for detection and purification of bovine herpesvirus 1 glycoproteins by their affinity for lectins is described. Wheat germ lectin, Limulus polyphemus lectin and concanavalin A, radioactive or biotin-labelled, have been used for the in vitro characterization of the glycoproteins. These lectins have also been used for affinity-purification of sets of glycoproteins. The procedure is easy, rapid, cheap and, when biotin is used, non-radioactive.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Glycoproteins / isolation & purification
  • Herpesvirus 1, Bovine / analysis*
  • Lectins
  • Molecular Weight
  • Viral Proteins / isolation & purification*

Substances

  • Glycoproteins
  • Lectins
  • Viral Proteins
  • bovine herpesvirus type-1 glycoproteins