Quantitative proteomic analysis of the fall armyworm saliva

Flor E Acevedo; Bruce A Stanley; Anne Stanley; Michelle Peiffer; Dawn S Luthe; Gary W Felton

doi:10.1016/j.ibmb.2017.06.001

Quantitative proteomic analysis of the fall armyworm saliva

Insect Biochem Mol Biol. 2017 Jul:86:81-92. doi: 10.1016/j.ibmb.2017.06.001. Epub 2017 Jun 4.

Authors

Flor E Acevedo¹, Bruce A Stanley², Anne Stanley³, Michelle Peiffer⁴, Dawn S Luthe⁵, Gary W Felton⁶

Affiliations

¹ Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, PA 16802, USA. Electronic address: floredith.acevedo@gmail.com.
² Section of Research Resources, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA. Electronic address: bas12@psu.edu.
³ Section of Research Resources, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA. Electronic address: astanley@psu.edu.
⁴ Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, PA 16802, USA. Electronic address: mlk101@psu.edu.
⁵ Department of Plant Science, Pennsylvania State University, 216 Agricultural Sciences and Industries Building, University Park, PA 16802, USA. Electronic address: dsl14@psu.edu.
⁶ Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, PA 16802, USA. Electronic address: gwf10@psu.edu.

PMID: 28591565
DOI: 10.1016/j.ibmb.2017.06.001

Abstract

Lepidopteran larvae secrete saliva on plant tissues during feeding. Components in the saliva may aid in food digestion, whereas other components are recognized by plants as cues to elicit defense responses. Despite the ecological and economical importance of these plant-feeding insects, knowledge of their saliva composition is limited to a few species. In this study, we identified the salivary proteins of larvae of the fall armyworm (FAW), Spodoptera frugiperda; determined qualitative and quantitative differences in the salivary proteome of the two host races-corn and rice strains-of this insect; and identified changes in total protein concentration and relative protein abundance in the saliva of FAW larvae associated with different host plants. Quantitative proteomic analyses were performed using labeling with isobaric tags for relative and absolute quantification followed by liquid chromatography-tandem mass spectrometry. In total, 98 proteins were identified (>99% confidence) in the FAW saliva. These proteins were further categorized into five functional groups: proteins potentially involved in (1) plant defense regulation, (2) herbivore offense, (3) insect immunity, (4) detoxification, (5) digestion, and (6) other functions. Moreover, there were differences in the salivary proteome between the FAW strains that were identified by label-free proteomic analyses. Thirteen differentially identified proteins were present in each strain. There were also differences in the relative abundance of eleven salivary proteins between the two FAW host strains as well as differences within each strain associated with different diets. The total salivary protein concentration was also different for the two strains reared on different host plants. Based on these results, we conclude that the FAW saliva contains a complex mixture of proteins involved in different functions that are specific for each strain and its composition can change plastically in response to diet type.

Keywords: Fall armyworm strains; Quantitative proteomics; Saliva; Sialome; iTRAQ.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Oryza
Proteome*
Proteomics
Saliva / chemistry*
Species Specificity
Spodoptera / chemistry*
Zea mays

Substances

Proteome