In vitro and in vivo antioxidant activities of inulin

Hong-Mei Shang; Hai-Zhu Zhou; Jun-Yan Yang; Ran Li; Hui Song; Hong-Xin Wu

doi:10.1371/journal.pone.0192273

In vitro and in vivo antioxidant activities of inulin

PLoS One. 2018 Feb 2;13(2):e0192273. doi: 10.1371/journal.pone.0192273. eCollection 2018.

Authors

Hong-Mei Shang^{1

2}, Hai-Zhu Zhou¹, Jun-Yan Yang¹, Ran Li¹, Hui Song³, Hong-Xin Wu⁴

Affiliations

¹ College of Animal Science and Technology, Jilin Agricultural University, Jilin, Changchun, China.
² Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, Jilin Agricultural University, Jilin, Changchun, China.
³ School of Life Sciences, Jilin Agricultural University, Jilin, Changchun, China.
⁴ Grassland Research Institute of CAAS, Neimenggu, Hohhot, China.

Abstract

This study was designed to investigate the in vitro and in vivo antioxidant activities of inulin. The in vitro assays demonstrated that the antioxidant activities of inulin, including the DPPH radical scavenging activity, ABTS scavenging activity and ferric reducing power, were weak and significantly lower than those of Vitamin C (P < 0.05). The influence of dietary supplementation with inulin on the antioxidant status of laying hens was evaluated with in vivo antioxidant assays. The results indicated that inulin supplementation quadratically improved the egg production rate of the laying hens (P < 0.01). The antioxidant enzyme activities in the serum, including SOD, CAT, and GSH-Px, and the total antioxidant capacity increased quadratically as inulin levels increased (P < 0.001). The levels of MDA in the serum decreased quadratically as inulin levels increased (P < 0.001). These findings suggest that inulin has the potential to improve the antioxidant status of laying hens.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants / pharmacology*
Chickens
Female
In Vitro Techniques
Inulin / pharmacology*

Substances

Antioxidants
Inulin

Grants and funding

This work was supported by the National Natural Science Foundation of China (31601972 to HMS) and Project funded by China Postdoctoral Science Foundation (2017M621224 to HMS).