Metabolism and prebiotics activity of anthocyanins from black rice (Oryza sativa L.) in vitro

Yongsheng Zhu; Hanju Sun; Shudong He; Qiuyan Lou; Min Yu; Mingming Tang; Lijun Tu

doi:10.1371/journal.pone.0195754

Metabolism and prebiotics activity of anthocyanins from black rice (Oryza sativa L.) in vitro

PLoS One. 2018 Apr 9;13(4):e0195754. doi: 10.1371/journal.pone.0195754. eCollection 2018.

Authors

Yongsheng Zhu¹, Hanju Sun¹, Shudong He^{1

2}, Qiuyan Lou¹, Min Yu¹, Mingming Tang¹, Lijun Tu¹

Affiliations

¹ School of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui, PR China.
² School of Food Science and Technology, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian, Liaoning, PR China.

Abstract

Anthocyanins are naturally active substances. In this study, anthocyanins from black rice were obtained by membrane filtration and column chromatography separation. Five anthocyanin monomers in black rice extract were identified by HPLC-MS/MS, and the major anthocyanin monomer (cyanidin-3-glucoside, C3G) was purified by preparative HPLC (Pre-HPLC). The proliferative effects of the anthocyanins on Bifidobacteria and Lactobacillus were investigated by determining the media pH, bacterial populations and metabolic products. After anaerobic incubation at 37 °C for 48 h, not only the pH of the media containing C3G was lower than that of the extract of black rice anthocyanin (BRAE), but the numbers of both Bifidobacteria and Lactobacillus were also significantly increased. Furthermore, hydroxyphenylpropionic, hydroxyphenylacetic, and hydroxybenzoic acids and other metabolites were detected by GC-MS in vitro. Our results revealed that the anthocyanins and anthocyanin monomers from black rice had prebiotic activity and they were metabolized into several small molecules by Bifidobacteria and Lactobacillus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthocyanins / isolation & purification
Anthocyanins / pharmacology*
Bifidobacterium / drug effects
Bifidobacterium / growth & development*
In Vitro Techniques
Lactobacillus / drug effects
Lactobacillus / growth & development*
Metabolome / drug effects*
Oryza / metabolism*
Prebiotics / analysis*

Substances

Anthocyanins
Prebiotics

Grants and funding

The authors are grateful for the financial support from the National Natural Science Foundation of China (No. 31771974, No. 31701524), the Anhui Provincial Natural Science Foundation (No. 1708085MC70), the Autumn Project of Hefei University of Technology (No. JZ2015QSJH0217), and the 61th Financial Grant from China Postdoctoral Science Foundation (No. 2017M611208). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.