TROSY pulse sequence for simultaneous measurement of the 15N R1 and {1H}-15N NOE in deuterated proteins

Paul A O'Brien; Arthur G Palmer 3rd

doi:10.1007/s10858-018-0181-6

TROSY pulse sequence for simultaneous measurement of the ¹⁵N R₁ and {¹H}-¹⁵N NOE in deuterated proteins

J Biomol NMR. 2018 Apr;70(4):205-209. doi: 10.1007/s10858-018-0181-6. Epub 2018 Apr 16.

Authors

Paul A O'Brien¹, Arthur G Palmer 3rd²

Affiliations

¹ Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY, 10032, USA.
² Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY, 10032, USA. agp6@columbia.edu.

Abstract

A TROSY-based NMR experiment is described for simultaneous measurement of the ¹⁵N longitudinal relaxation rate constant R₁ and the {¹H}-¹⁵N nuclear Overhauser enhancement. The experiment is based on the observation that the TROSY mixing pulse sequence element symmetrically exchanges ¹H and ¹⁵N magnetizations. The accuracy of the proposed technique is validated by comparison to independent measurements of both relaxation parameters for the protein ubiquitin. The simultaneous experiment is approximately 20-33% shorter than conventional sequential measurements.

Keywords: Dynamics; Longitudinal relaxation; Nuclear Overhauser enhancement; Protein; Spin–lattice relaxation; TROSY.

MeSH terms

Deuterium
Magnetic Phenomena
Nitrogen Isotopes*
Nuclear Magnetic Resonance, Biomolecular / methods*
Proteins / chemistry*
Protons*
Ubiquitin / chemistry

Substances

Nitrogen Isotopes
Nitrogen-15
Proteins
Protons
Ubiquitin
Deuterium

Abstract

MeSH terms

Substances

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